Potensi Vaksin Lasota Terhadap Tantangan Virus Tetelo atau Newcastle Disease Velogenik Lapang

Plaque formation of LaSota pathogenic strain of Newcastle disease virus adapted in chick embryo fibroblast cells

In order to adapt LaSota strain of Newcastle disease virus (NDV) on chick embryo fibroblast (CEF) cells, 0.1 ml from LaSota vaccine produced in Razi vaccine and serum research institute inoculated to the CEF cells and was passaged five times in the CEF grown in Eagle’s minimum essential medium (MEM). First to third passages were blind but in fourth passage cytopathic effect of virus was observe...

Newcastle disease virus LaSota strain kills human pancreatic cancer cells in vitro with high selectivity.

CONTEXT Pancreatic cancer is highly resistant to treatment. Previously, we showed that Newcastle disease virus (NDV) strain 73-T was highly cytotoxic to a range of tumor types in vitro and in vivo but the effects of NDV on pancreatic tumors are unknown. We determined the cytotoxicity of the lentogenic LaSota strain of NDV (NDV-LS) toward 7 different human pancreatic tumor cell lines and 4 norma...

Historical note on the origin of the LaSota strain of Newcastle disease virus.

Comparative Efficacy of Five Different Brands of Commercial Newcastle Disease Lasota Virus Vaccines in Broilers

Five commercial LaSota strain Newcastle disease (ND) vaccines namely A, B, C, D and E were evaluated for their potency, efficacy, thermostability and influence on productivity in broilers. A 3-log10 difference of EID50 and two-to-eight fold difference of HA activity was found among the various vaccines tested. One hundred and fifty day-old broiler chicks were divided into six equal groups tagge...

Clone Purification, Characterization and Standardization of LaSota Strain for Developing a Live Vaccine against Newcastle Disease Virus

Newcastle disease (ND) is one of the most serious illnesses of chickens. Live vaccines are widely used to prevent chicken from the disease all over the world. To access the effective and potentiate ND vaccine, a homogenous subpopulation from LaSota strain was selected following cultivation of the virus on primary chicken embryofibroblast (CEF) cells. Pathogenicity indices and molecular characte...